پروژه کنوا - 103926

میتونید امروز چند تا اسلاید درست کنید واسم با canava فقط باید ادیت شه متن امادست 6صفحه Canava.com Materials and methods of ApoE- modified solid lipid nano particles: – Preparation & Characterization of SLN-mApoE – In vitro: Cytotoxicity & Uptake by Endothelial Cells _time dependent nano particle-cell interaction _intracellular distribution of SLN by confocal microscopy in hCMEC/D3 _pathway involved in the cellular uptake of SLN-mAPoE _endothelial permeability of radiolabelled SLN _Animals _In Vivo administration of SLN _Bronchoalveolar lavage fluid analysis _statistical analysis این اسلاید اولش SLN prepration: • Preparation: warm oil/water microemulsion → cold water dispersion → SLN • Surface functionalization: • incorporate maleimide-PEG-lipid (DPM) in SLN • covalent coupling with thiol-modified ApoE peptide (mApoE) • Purification: tangential ultrafiltration • Characterization: size, PDI, ζ-potential, coupling efficiency Methods: • Warm microemulsion technique • Thiol–maleimide coupling (DPM–mApoE) • Dynamic light scattering (for size, PDI, zeta) • Fluorescence spectroscopy of tryptophan (coupling efficiency) 3398